マブチ　ミユキ   MIYUKI MABUCHI   馬渕　美雪    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2015/07
形態種別	学術雑誌
査読	査読あり
標題	Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506.
執筆形態	共著
掲載誌名	Bioorganic & medicinal chemistry letters
掲載区分	国外
巻・号・頁	25(14),pp.2788-92
著者・共著者	Mabuchi Miyuki, Shimizu Tadashi, Ueda Masahiro, Mitamura Kuniko, Ikegawa Shigeo, Tanaka Akito
概要	Solid materials for affinity resins bearing long PEG spacers between a functional group used for immobilization of a bio-active compound and the solid surface were synthesized to capture not only small target proteins but also large and/or complex target proteins. Solid materials with PEG1000 or PEG2000 as spacers, which bear a benzenesulfonamide derivative, exhibited excellent selectivity between the specific binding protein carbonic anhydrase type II (CAII) and non-specific ones. These materials also exhibited efficacy in capturing a particular target at a maximal amount. Affinity resins using solid materials with PEG1000 or PEG2000 spacers, bear a FK506 derivative, successfully captured the whole target complex of specific binding proteins at the silver staining level, while all previously known affinity resins with solid materials failed to achieve this objective. These novel affinity resins captured other specific binding proteins such as dynamin and neurocalcin δ as well.
DOI	10.1016/j.bmcl.2015.05.014
ISSN	1464-3405
PMID	26025877