マブチ　ミユキ   馬渕　美雪    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	日本語
発行・発表の年月	2010/12
形態種別	学術雑誌
査読	査読あり
標題	Selective elution of target protein from affinity resins by a simple reductant with a thiol group.
執筆形態	共著
掲載誌名	Bioorganic & medicinal chemistry letters
掲載区分	国外
巻・号・頁	20(24),7361-4頁
著者・共著者	Mabuchi Miyuki, Haramura Masayuki, Shimizu Tadashi, Nishizaki Tomoyuki, Tanaka Akito
概要	We have made a chance discovery of selective elution of a specific binding protein from affinity resins by mixing them with aqueous solutions of a widely used reductant, 2-mercaptoethanol (2ME), under mild conditions. Our studies suggest this phenomenon would be generic, and could be a powerful method for identification of a specific binding protein. We here exhibit the experimental conditions and successful examples in which target proteins of benzensulfonamide and FK506 were selectively eluted from affinity resins bearing these compounds, while non-specific ones remained.
DOI	10.1016/j.bmcl.2010.10.053
ISSN	1464-3405
PMID	21067925