タカラ　コウジ   髙良　恒史    所属   兵庫医科大学  薬学部 医療薬学科    職種   准教授
言語種別	日本語
発行・発表の年月	2010
形態種別	学術雑誌
査読	査読あり
標題	Characterization, in vitro cytotoxicity and cellular accumulation of paclitaxel-loaded lipid nano-emulsions.
執筆形態	共著
掲載誌名	Journal of microencapsulation
掲載区分	国外
巻・号・頁	27(5),453-9頁
著者・共著者	Takegami Shigehiko, Takara Kohji, Tanaka Shiori, Yamamoto Kazuhiro, Hori Masahiro, Yokoyama Teruyoshi, Kitade Tatsuya
概要	Lipid nano-emulsions (LNEs) having a mean droplet size of approximately 50 nm were investigated as drug carriers for paclitaxel (TXL) to achieve its satisfactory loadings and to develop a pharmaceutically acceptable alternative to the current formulation, Taxol. TXL was incorporated into the LNEs at 2.0 mg/ml without changes in particle size or drug precipitation. In the cytotoxicity study, TXL-loaded LNEs had cytotoxicity to HeLa cells equivalent to that of TXL alone; the 50% growth inhibitory concentrations (IC(50)) of TXL-loaded LNEs and TXL alone were 1.53 +/- 0.23 nM and 1.76 +/- 0.08 nM, respectively. However, a cellular accumulation study using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a fluorescent probe showed that the accumulation of DPH-loaded LNEs in HeLa cells was remarkably lower than that of DPH alone. These results indicated that LNEs were a useful vehicle for TXL, even though LNEs themselves could not be efficiently accumulated in HeLa cells.
DOI	10.3109/02652040903515482
ISSN	1464-5246
PMID	20113171