ハギハラ カナコ
Hagihara Kanako
萩原 加奈子 所属 兵庫医科大学 薬学部 医療薬学科 職種 助教 |
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言語種別 | 英語 |
発行・発表の年月 | 2009/05 |
形態種別 | 学術雑誌 |
査読 | 査読あり |
標題 | Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast. |
執筆形態 | 共著 |
掲載誌名 | Molecular biology of the cell |
掲載区分 | 国外 |
巻・号・頁 | 20(9),pp.2473-85 |
著者・共著者 | Satoh Ryosuke, Morita Takahiro, Takada Hirofumi, Kita Ayako, Ishiwata Shunji, Doi Akira, Hagihara Kanako, Taga Atsushi, Matsumura Yasuhiro, Tohda Hideki, Sugiura Reiko |
概要 | Here, we isolated Nrd1, an RNA-binding protein with RNA-recognition motifs, as a multicopy suppressor of cdc4 mutants. Notably, we demonstrated that Nrd1 binds and stabilizes Cdc4 mRNA, thereby suppressing the cytokinesis defects of the cdc4 mutants. Importantly, Pmk1 mitogen-activated protein kinase (MAPK) directly phosphorylates Nrd1, thereby negatively regulating the binding activity of Nrd1 to Cdc4 mRNA. In addition, we demonstrated the cell cycle-dependent regulation of Pmk1/Nrd1 signaling. Together, our results indicate that Nrd1 plays a role in the regulation of Cdc4 mRNA stability; moreover, our study is the first to demonstrate the posttranscriptional regulation of myosin expression by MAPK signaling. |
DOI | 10.1091/mbc.e08-09-0893 |
ISSN | 1939-4586 |
PMID | 19279143 |