ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2017/05
形態種別	学術雑誌
査読	査読あり
標題	Spatial regulation of the KH domain RNA-binding protein Rnc1 mediated by a Crm1-independent nuclear export system in Schizosaccharomyces pombe.
執筆形態	共著
掲載誌名	Molecular microbiology
掲載区分	国外
巻・号・頁	104(3),pp.428-448
著者・共著者	Satoh Ryosuke, Matsumura Yasuhiro, Tanaka Akitomo, Takada Makoto, Ito Yuna, Hagihara Kanako, Inari Masahiro, Kita Ayako, Fukao Akira, Fujiwara Toshinobu, Hirai Shinya, Tani Tokio, Sugiura Reiko
概要	Here, we analyzed the spatial regulation of Rnc1 and discovered a putative nuclear export signal (NES)Rnc1 , which dictates the cytoplasmic localization of Rnc1 in a Crm1-independent manner. Intriguingly, the Rnc1 NES mutant destabilized Pmp1 mRNA, suggesting the functional importance of the Rnc1 cytoplasmic localization. Mutation in Rae1, but not Mex67 deletion or overproduction, induced Rnc1 accumulation in the nucleus, suggesting that Rnc1 is exported from the nucleus to the cytoplasm via the mRNA export pathway involving Rae1. Importantly, mutations in the Rnc1 KH-domains abolished the mRNA-binding ability and induced nuclear localization, suggesting that Rnc1 may be exported from the nucleus together with its target mRNAs. Collectively, the functional Rae1-dependent mRNA export system may influence the cytoplasmic localization and function of Rnc1.
DOI	10.1111/mmi.13636
ISSN	1365-2958
PMID	28142187