ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2014/04
形態種別	学術雑誌
査読	査読あり
標題	FTY720 stimulated ROS generation and the Sty1/Atf1 signaling pathway in the fission yeast Schizosaccharomyces pombe.
執筆形態	共著
掲載誌名	Genes to cells : devoted to molecular & cellular mechanisms
掲載区分	国外
巻・号・頁	19(4),pp.325-37
著者・共著者	◎Hagihara Kanako, Mizukura Aya, Kitai Yuki, Yao Mariko, Ishida Kouki, Kita Ayako, Kunoh Tatsuki, Masuko Takashi, Matzno Sumio, Chiba Kenji, Sugiura Reiko
概要	Fingolimod hydrochloride (FTY720) is the first-in-class immune modulator known as sphingosine 1-phosphate (S1P) receptor agonists. FTY720 stimulated the CRE responses in a concentration-dependent manner, which was markedly reduced by deletion of the components of the Spc1/Sty1 MAPK pathway. The blockade of ROS production by NAC (N-acetyl-L-cysteine) significantly reversed the FTY720-induced ROS accumulation, subsequent activation of the Spc1/Sty1 MAPK pathway, and inhibition of cell proliferation. Cells lacking the components of the Spc1/Sty1 MAPK exhibited higher sensitivity to FTY720 and higher ROS levels upon FTY720 treatment than in wild-type cells. Thus, our results demonstrate the usefulness of fission yeast for elucidating the FTY720-mediated signaling pathways involving ROS.
DOI	10.1111/gtc.12134
ISSN	1365-2443
PMID	24506481