ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2015/02
形態種別	学術雑誌
査読	査読あり
標題	Spatial control of calcineurin in response to heat shock in fission yeast.
執筆形態	共著
掲載誌名	Genes to cells : devoted to molecular & cellular mechanisms
掲載区分	国外
巻・号・頁	20(2),pp.95-107
著者・共著者	Higa Mari, Kita Ayako, Hagihara Kanako, Kitai Yuki, Doi Akira, Nagasoko Rie, Satoh Ryosuke, Sugiura Reiko
概要	Here, we show that heat shock stimulates calcineurin-dependent nuclear translocation of Prz1 and CDRE-dependent gene expression. Notably, calcineurin exhibited a dramatic change in subcellular localization, translocating from diffuse cytoplasmic to dot-like structures on heat shock. The calcineurin dots colocalized with Dcp2 or Pabp, the constituent of P-bodies or stress granules, respectively, thus suggesting that calcineurin is a component of RNA granules under heat shock. Importantly, the calcineurin inhibitor FK506 markedly inhibited the accumulation of calcineurin granules, whereas the constitutively active calcineurin strongly accumulated in the granules on heat shock, suggesting that phosphatase activity is important for calcineurin localization. Notably, the depletion of calcineurin induced a rapid appearance of Nrd1- and Pabp-positive RNA granules. The possible roles of calcineurin in response to heat shock will be discussed.
DOI	10.1111/gtc.12203
ISSN	1365-2443
PMID	25529221