ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2016/08
形態種別	学術雑誌
査読	査読あり
標題	Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by controlling the intracellular localization of the MAPKKK Mkh1.
執筆形態	共著
掲載誌名	Journal of cell science
掲載区分	国外
巻・号・頁	129(16),pp.3189-202
著者・共著者	Kanda Yuki, Satoh Ryosuke, Matsumoto Saki, Ikeda Chisato, Inutsuka Natsumi, Hagihara Kanako, Matzno Sumio, Tsujimoto Sho, Kita Ayako, Sugiura Reiko
概要	Here, we show that Skb5 serves as an inhibitor of Pmk1 MAPK signaling activation by downregulating Mkh1 localization to cell tips through its interaction with the SH3 domain. Consistent with this, the Mkh1(3PA) mutant protein, with impaired Skb5 binding, remained in the cell tips, even when Skb5 was overproduced. Intriguingly, Skb5 needs Mkh1 to localize to the growing ends as Mkh1 deletion and disruption of Mkh1 binding impairs Skb5 localization. Deletion of Pck2, an upstream activator of Mkh1, impaired the cell tip localization of Mkh1 and Skb5 as well as the Mkh1-Skb5 interaction. Interestingly, both Pck2 and Mkh1 localized to the cell tips at the G1/S phase, which coincided with Pmk1 MAPK activation. Taken together, Mkh1 localization to cell tips is important for transmitting upstream signaling to Pmk1, and Skb5 spatially regulates this process.
DOI	10.1242/jcs.188854
ISSN	1477-9137
PMID	27451356