ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2009/05
形態種別	学術雑誌
査読	査読あり
標題	Role of the RNA-binding protein Nrd1 and Pmk1 mitogen-activated protein kinase in the regulation of myosin mRNA stability in fission yeast.
執筆形態	共著
掲載誌名	Molecular biology of the cell
掲載区分	国外
巻・号・頁	20(9),pp.2473-85
著者・共著者	Satoh Ryosuke, Morita Takahiro, Takada Hirofumi, Kita Ayako, Ishiwata Shunji, Doi Akira, Hagihara Kanako, Taga Atsushi, Matsumura Yasuhiro, Tohda Hideki, Sugiura Reiko
概要	Here, we isolated Nrd1, an RNA-binding protein with RNA-recognition motifs, as a multicopy suppressor of cdc4 mutants. Notably, we demonstrated that Nrd1 binds and stabilizes Cdc4 mRNA, thereby suppressing the cytokinesis defects of the cdc4 mutants. Importantly, Pmk1 mitogen-activated protein kinase (MAPK) directly phosphorylates Nrd1, thereby negatively regulating the binding activity of Nrd1 to Cdc4 mRNA. In addition, we demonstrated the cell cycle-dependent regulation of Pmk1/Nrd1 signaling. Together, our results indicate that Nrd1 plays a role in the regulation of Cdc4 mRNA stability; moreover, our study is the first to demonstrate the posttranscriptional regulation of myosin expression by MAPK signaling.
DOI	10.1091/mbc.e08-09-0893
ISSN	1939-4586
PMID	19279143