ハギハラ　カナコ   Hagihara Kanako   萩原　加奈子    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2013
形態種別	学術雑誌
査読	査読あり
標題	Fingolimod (FTY720) stimulates Ca(2+)/calcineurin signaling in fission yeast.
執筆形態	共著
掲載誌名	PloS one
掲載区分	国外
巻・号・頁	8(12),pp.e81907
著者・共著者	◎Hagihara Kanako, Kita Ayako, Mizukura Aya, Yao Mariko, Kitai Yuki, Kunoh Tatsuki, Masuko Takashi, Matzno Sumio, Chiba Kenji, Sugiura Reiko
概要	Fingolimod hydrochloride (FTY720) is the first in class of sphingosine 1-phosphate (S1P) receptor modulator approved to treat multiple sclerosis via down-regulation of G protein-coupled S1P receptor 1 by its phosphorylated form (FTY720-P). To gain further insights into molecular mechanisms of FTY720 action, the effect of FTY720 on Ca(2+) signaling in fission yeast was analyzed. The addition of Ca(2+) enhanced the sensitivity induced by FTY720, and mutants lacking genes required for calcium homeostasis, including calcineurin and its downstream transcription factor, Ppb1-responsive zinc finger protein (Prz1), were hypersensitive to FTY720 and CaCl2. Notably, the FTY720-mediated Ca(2+) influx and calcineurin activation were reduced markedly by the deletion of yam8 (+) or cch1 (+) encoding putative subunits of a Ca(2+) channel. These results suggest that the FTY720-stimulated Ca(2+)/calcineurin signaling activation partly involves the Yam8/Cch1 channel in fission yeast.
DOI	10.1371/journal.pone.0081907
ISSN	1932-6203
PMID	24312601