マブチ　ミユキ   MIYUKI MABUCHI   馬渕　美雪    所属   兵庫医科大学  薬学部 医療薬学科    職種   助教
言語種別	英語
発行・発表の年月	2015/08
形態種別	学術雑誌
査読	査読あり
標題	Identification and purification of target protein using affinity resin bearing a photo-labile linker.
執筆形態	共著
掲載誌名	Bioorganic & medicinal chemistry letters
掲載区分	国外
巻・号・頁	25(16),pp.3373-7
著者・共著者	Mabuchi Miyuki, Shimizu Tadashi, Haramura Masayuki, Tanaka Akito
概要	This Letter presents an effective method for the identification of target proteins of bioactive compounds such as drugs, natural products, and intrinsic ligands, using an affinity resin. The application of a photo-labile linker to an affinity resin enabled the selective elution of a target protein by irradiation for a short duration at 4 °C while leaving a large amount of non-specific binding protein on the resin. We have named this method the 'STEAP' method (selective target elution from affinity resins with photo-labile linker). Only a target protein that can bind the bioactive compound, the so-called 'active' protein, is eluted by the selective cleavage of the linker between the solid matrix and the target compound, and therefore, it is worth considering the potential of this method for the hyper-purification of proteins.
DOI	10.1016/j.bmcl.2015.05.023
ISSN	1464-3405
PMID	26099537